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staining intensity  (Vector Laboratories)


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    Structured Review

    Vector Laboratories staining intensity
    Staining Intensity, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 12397 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+intensity/DAB+Peroxidase+(HRP)+Substrate+Kit+(with+Nickel)%2C+3%2C3%E2%80%99-diaminobenzidine/pm41215730-472-51-45
    Average 96 stars, based on 12397 article reviews
    staining intensity - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Amplification:

    Article Title: Impaired nuclear PTEN function drives macrocephaly, lymphadenopathy and late-onset cancer in PTEN hamartoma tumour syndrome.
    Article Snippet: Primary antibodies were detected with biotinylated secondary antibodies (Donkey Anti-Mouse IgG Biotinylated Antibody, R&D Systems) diluted 1:300 in blocking buffer for 1 h at RT. .. The signal D is ea se M o d el s & M ec h an is m s was amplified using the Vectastain ABC Kit HRP kit (Vector Laboratories, PK-6100), according to the manufacturer’s instructions and developed using a DAB Peroxidase (HRP) Substrate Kit (Vector Laboratories, SK-4100) until the desired staining intensity was reached. .. Floating sections were mounted on SuperFrost Plus slides (Thermo Fisher Scientific) in 0.2% pork skin gelatine dissolved in 50 mM Tris-HCl pH 7.4, dried at RT overnight, and dehydrated in ethanol and xylene before mounting with DPX mounting medium (Merck).

    Staining:

    Article Title: Impaired nuclear PTEN function drives macrocephaly, lymphadenopathy and late-onset cancer in PTEN hamartoma tumour syndrome.
    Article Snippet: Primary antibodies were detected with biotinylated secondary antibodies (Donkey Anti-Mouse IgG Biotinylated Antibody, R&D Systems) diluted 1:300 in blocking buffer for 1 h at RT. .. The signal D is ea se M o d el s & M ec h an is m s was amplified using the Vectastain ABC Kit HRP kit (Vector Laboratories, PK-6100), according to the manufacturer’s instructions and developed using a DAB Peroxidase (HRP) Substrate Kit (Vector Laboratories, SK-4100) until the desired staining intensity was reached. .. Floating sections were mounted on SuperFrost Plus slides (Thermo Fisher Scientific) in 0.2% pork skin gelatine dissolved in 50 mM Tris-HCl pH 7.4, dried at RT overnight, and dehydrated in ethanol and xylene before mounting with DPX mounting medium (Merck).



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    Image Search Results


    Distribution trend of TOP2A high expression group and low expression group

    Journal: Discover Oncology

    Article Title: The analysis of TOP2A as a potential target for predicting the prognosis of brain glioma in the TCGA database and in single-center study

    doi: 10.1007/s12672-025-04051-4

    Figure Lengend Snippet: Distribution trend of TOP2A high expression group and low expression group

    Article Snippet: D Box plot expression of TOP2A staining score in low-grade glioma and high-grade glioma. ( P < 0.05 is considered statistically significant) Fig. 8 Representative IHC of TOP2A staining intensity expression in glioma (Images were taken by OLYMPUS SC180 microscope.)

    Techniques: Expressing

    Survival prognostic model. A K-M curve, the survival rate of TOP2A high standard group was significantly worse than that of low expression group. B The AUC value of the ROC model was greater than 0.7, indicating that the accuracy of the prognostic model was good ( P < 0.05 is considered statistically significant)

    Journal: Discover Oncology

    Article Title: The analysis of TOP2A as a potential target for predicting the prognosis of brain glioma in the TCGA database and in single-center study

    doi: 10.1007/s12672-025-04051-4

    Figure Lengend Snippet: Survival prognostic model. A K-M curve, the survival rate of TOP2A high standard group was significantly worse than that of low expression group. B The AUC value of the ROC model was greater than 0.7, indicating that the accuracy of the prognostic model was good ( P < 0.05 is considered statistically significant)

    Article Snippet: D Box plot expression of TOP2A staining score in low-grade glioma and high-grade glioma. ( P < 0.05 is considered statistically significant) Fig. 8 Representative IHC of TOP2A staining intensity expression in glioma (Images were taken by OLYMPUS SC180 microscope.)

    Techniques: Expressing

    TOP2A-related gene analysis. A Heat map of genes with strong correlation with TOP2A, red represents positive correlation and blue represents negative correlation. B Correlation circle plot of the above genes, red represents positive correlation and green represents negative correlation. C Biological function analysis of the above genes (GO analysis). D , E Biological function analysis of the above genes (KEGG analysis) ( P < 0.05 is considered statistically significant)

    Journal: Discover Oncology

    Article Title: The analysis of TOP2A as a potential target for predicting the prognosis of brain glioma in the TCGA database and in single-center study

    doi: 10.1007/s12672-025-04051-4

    Figure Lengend Snippet: TOP2A-related gene analysis. A Heat map of genes with strong correlation with TOP2A, red represents positive correlation and blue represents negative correlation. B Correlation circle plot of the above genes, red represents positive correlation and green represents negative correlation. C Biological function analysis of the above genes (GO analysis). D , E Biological function analysis of the above genes (KEGG analysis) ( P < 0.05 is considered statistically significant)

    Article Snippet: D Box plot expression of TOP2A staining score in low-grade glioma and high-grade glioma. ( P < 0.05 is considered statistically significant) Fig. 8 Representative IHC of TOP2A staining intensity expression in glioma (Images were taken by OLYMPUS SC180 microscope.)

    Techniques:

    Correlation analysis of TOP2A and immune cells and genes. A Correlation analysis between TOP2A and immune cells, Macrophages M0 and TOP2A expression have a certain correlation. B The correlation between TOP2A and immune genes was analyzed, and the correlation with CD276 was the strongest. C Expression of TOP2A and trend chart of various immune data ( P < 0.05 is considered statistically significant)

    Journal: Discover Oncology

    Article Title: The analysis of TOP2A as a potential target for predicting the prognosis of brain glioma in the TCGA database and in single-center study

    doi: 10.1007/s12672-025-04051-4

    Figure Lengend Snippet: Correlation analysis of TOP2A and immune cells and genes. A Correlation analysis between TOP2A and immune cells, Macrophages M0 and TOP2A expression have a certain correlation. B The correlation between TOP2A and immune genes was analyzed, and the correlation with CD276 was the strongest. C Expression of TOP2A and trend chart of various immune data ( P < 0.05 is considered statistically significant)

    Article Snippet: D Box plot expression of TOP2A staining score in low-grade glioma and high-grade glioma. ( P < 0.05 is considered statistically significant) Fig. 8 Representative IHC of TOP2A staining intensity expression in glioma (Images were taken by OLYMPUS SC180 microscope.)

    Techniques: Expressing

    Representative IHC of TOP2A staining intensity expression and box plot expression of TOP2A staining score in glioma. A TOP2A staining intensity was weakly positive in glioma. (Magnification, x100; EnVision two-step). B TOP2A staining intensity was moderately positive in glioma. (Magnification, x100; EnVision two-step). C TOP2A staining intensity was strongly positive in glioma. (Magnification, x100; EnVision two-step). D Box plot expression of TOP2A staining score in low-grade glioma and high-grade glioma. ( P < 0.05 is considered statistically significant)

    Journal: Discover Oncology

    Article Title: The analysis of TOP2A as a potential target for predicting the prognosis of brain glioma in the TCGA database and in single-center study

    doi: 10.1007/s12672-025-04051-4

    Figure Lengend Snippet: Representative IHC of TOP2A staining intensity expression and box plot expression of TOP2A staining score in glioma. A TOP2A staining intensity was weakly positive in glioma. (Magnification, x100; EnVision two-step). B TOP2A staining intensity was moderately positive in glioma. (Magnification, x100; EnVision two-step). C TOP2A staining intensity was strongly positive in glioma. (Magnification, x100; EnVision two-step). D Box plot expression of TOP2A staining score in low-grade glioma and high-grade glioma. ( P < 0.05 is considered statistically significant)

    Article Snippet: D Box plot expression of TOP2A staining score in low-grade glioma and high-grade glioma. ( P < 0.05 is considered statistically significant) Fig. 8 Representative IHC of TOP2A staining intensity expression in glioma (Images were taken by OLYMPUS SC180 microscope.)

    Techniques: Staining, Expressing

    Representative IHC of TOP2A staining intensity expression in glioma (Images were taken by OLYMPUS SC180 microscope.) A A low-grade glioma tissue showing low TOP2A protein expression (magnification, x10 left and x40 right). B A high-grade glioma tissue showing high TOP2A protein expression (magnification, x10 left and x40 right)

    Journal: Discover Oncology

    Article Title: The analysis of TOP2A as a potential target for predicting the prognosis of brain glioma in the TCGA database and in single-center study

    doi: 10.1007/s12672-025-04051-4

    Figure Lengend Snippet: Representative IHC of TOP2A staining intensity expression in glioma (Images were taken by OLYMPUS SC180 microscope.) A A low-grade glioma tissue showing low TOP2A protein expression (magnification, x10 left and x40 right). B A high-grade glioma tissue showing high TOP2A protein expression (magnification, x10 left and x40 right)

    Article Snippet: D Box plot expression of TOP2A staining score in low-grade glioma and high-grade glioma. ( P < 0.05 is considered statistically significant) Fig. 8 Representative IHC of TOP2A staining intensity expression in glioma (Images were taken by OLYMPUS SC180 microscope.)

    Techniques: Staining, Expressing, Microscopy

    Survival prognostic model in real world clinical samples. A K-M curve, the survival rate of TOP2A high standard group was significantly worse than that of low expression group. B The AUC value of the ROC model was greater than 0.7, indicating that the accuracy of the prognostic model was good ( P < 0.05 is considered statistically significant)

    Journal: Discover Oncology

    Article Title: The analysis of TOP2A as a potential target for predicting the prognosis of brain glioma in the TCGA database and in single-center study

    doi: 10.1007/s12672-025-04051-4

    Figure Lengend Snippet: Survival prognostic model in real world clinical samples. A K-M curve, the survival rate of TOP2A high standard group was significantly worse than that of low expression group. B The AUC value of the ROC model was greater than 0.7, indicating that the accuracy of the prognostic model was good ( P < 0.05 is considered statistically significant)

    Article Snippet: D Box plot expression of TOP2A staining score in low-grade glioma and high-grade glioma. ( P < 0.05 is considered statistically significant) Fig. 8 Representative IHC of TOP2A staining intensity expression in glioma (Images were taken by OLYMPUS SC180 microscope.)

    Techniques: Expressing

    (A–D) The OS curve of the core genes between the 2 risk groups; (E) immunohistochemical staining for 3 HLMRGs in the normal oral mucosa tissue and HNC; (F) correlation between HS3ST1, CYP27A1, STC2, and EGLN2 levels and immune cell infiltration based on CIBERSORT. CIBERSORT = Cell-type Identification By Estimating Relative Subsets Of RNA Transcripts, HLMRG = hypoxia- and lactate metabolism-related genes, HNC = head and neck cancer, OS = overall survival.

    Journal: Medicine

    Article Title: Development and experimental verification of a prognosis model for hypoxia- and lactate metabolism-associated genes in HNSCC

    doi: 10.1097/MD.0000000000042665

    Figure Lengend Snippet: (A–D) The OS curve of the core genes between the 2 risk groups; (E) immunohistochemical staining for 3 HLMRGs in the normal oral mucosa tissue and HNC; (F) correlation between HS3ST1, CYP27A1, STC2, and EGLN2 levels and immune cell infiltration based on CIBERSORT. CIBERSORT = Cell-type Identification By Estimating Relative Subsets Of RNA Transcripts, HLMRG = hypoxia- and lactate metabolism-related genes, HNC = head and neck cancer, OS = overall survival.

    Article Snippet: We further evaluated the immunohistochemical images and staining intensity of HLMRGs in HNSCC and normal tissues in the Human Protein Atlas (HPA) database ( https://www.proteinatlas.org/ ).

    Techniques: Immunohistochemical staining, Staining